Venom Purification
Mostrando 1-12 de 80 artigos, teses e dissertações.
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1. Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
Abstract Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein f
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/02/2019
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2. Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Abstract Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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3. Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Abstract Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I. Methods and Results: For its purification, three cons
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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4. Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening
Abstract Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms shou
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 22/02/2018
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5. BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom
Abstract Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas.
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/02/2018
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6. Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Abstract Background Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom ant
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 22/09/2016
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7. Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom
Abstract Background The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The objective of our research was to isolate and identify the com
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 31/12/2015
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8. Antiproliferative activity of marine stingray Dasyatis sephenvenom on human cervical carcinoma cell line
AbstractBackgroundVenoms comprise mixtures of numerous bioactive compounds that have a wide range of pharmacologic actions. Toxins from venomous animals have attracted the attention of researchers because of their affinity for primary sites responsible for lethality and their efficacy at extremely low concentrations. The venoms of marine stingrays have not b
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 10/11/2015
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9. Snake venom galactoside-binding lectins: a structural and functional overview
AbstractSnake venom galactoside-binding lectins (SVgalLs) comprise a class of toxins capable of recognizing and interacting with terminal galactoside residues of glycans. In the past 35 years, since the first report on the purification of thrombolectin from Bothrops atrox snake venom, several SVgalLs from Viperidae and Elapidae snake families have been descr
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 20/10/2015
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10. Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specim
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
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11. Purification and characterization of a hyaluronidase from venom of the spider Vitalius dubius (Araneae, Theraphosidae)
BackgroundVenom hyaluronidase (Hyase) contributes to the diffusion of venom from the inoculation site. In this work, we purified and characterized Hyase from the venom of Vitalius dubius (Araneae, Theraphosidae), a large theraphosid found in southeastern Brazil. Venom obtained by electrical stimulation of adult male and female V. dubius was initially fractio
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 04/02/2014
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12. CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake ven
Rev. Inst. Med. trop. S. Paulo. Publicado em: 2014-01