Vigs
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Construção de um vetor viral, baseado no DNA-A do Tomato rugose mosaic virus (ToRMV), para a indução de silenciamento gênico em plantas hospedeiras / Construction of a viral vector based on DNA-A of Tomato rugose mosaic virus (ToRMV) for induction of gene silencing in host plants
Os begomovírus pertencem à família Geminiviridae, que inclui vírus com genoma composto por uma ou duas moléculas de DNA circular de fita simples, encapsidado em partículas icosaédricas geminadas. Os begomovírus são transmitidos pela mosca branca Bemisia tabaci e causam doenças de importância econômica em diversas culturas, principalmente em regi�
Publicado em: 2009
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2. Identificação de genes potencialmente envolvidos na interação tomateiro - Potyvirus / Identification of genes potentially involved in the interaction tomato - Potyvirus
During co-evolution between virus and host, a complex interaction has been developed involving several mechanisms of pathogen attack and host defense. Host defense responses cause up- and downward shifts in gene expression. However, the effects of viral infection in the hosts gene expression profile are still poorly understood. With the objective of identify
Publicado em: 2006
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3. Initiation and maintenance of virus-induced gene silencing
The phytoene desaturase (PDS) gene of Nicotiana benthamiana was silenced in plants infected with potato virus X (PVX) vectors carrying PDS inserts, and a green fluorescent protein (GFP) transgene was silenced in plants infected with PVX-GFP. This virus-induced gene silencing (VIGS) is post-transcriptional and cytoplasmic because it is targeted against exons
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4. Potato Virus X-Induced Gene Silencing in Leaves and Tubers of Potato1
Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously described potato virus X (PVX) VIGS vector has been shown to trigger silencing in the permissive host Nic
American Society of Plant Biologists.
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5. A Visual Reporter System for Virus-Induced Gene Silencing in Tomato Fruit Based on Anthocyanin Accumulation1[C][W]
Virus-induced gene silencing (VIGS) is a powerful tool for reverse genetics in tomato (Solanum lycopersicum). However, the irregular distribution of the effects of VIGS hampers the identification and quantification of nonvisual phenotypes. To overcome this limitation, a visually traceable VIGS system was developed for fruit, comprising two elements: (1) a tr
American Society of Plant Biologists.
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6. Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway1
Virus-induced gene silencing (VIGS) is an important tool for the analysis of gene function in plants. In VIGS, viruses engineered to carry sequences derived from plant gene transcripts activate the host's sequence-specific RNA degradation system. This mechanism targets the RNAs of the viral genome for degradation, and as the virus contains transcribed plant
American Society of Plant Biologists.
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7. Nicotiana benthamiana gp91phox Homologs NbrbohA and NbrbohB Participate in H2O2 Accumulation and Resistance to Phytophthora infestans
Active oxygen species (AOS) are responsible for triggering defense responses in plants. Respiratory burst oxidase homologs (rboh genes) have been implicated in AOS generation. We have isolated two rboh cDNAs, NbrbohA and NbrbohB, from Nicotiana benthamiana leaves. NbrbohA was expressed constitutively at a low level and the transcripts were increased after me
American Society of Plant Biologists.
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8. Efficient Virus-Induced Gene Silencing in Roots Using a Modified Tobacco Rattle Virus Vector1[w]
Due to their capability of eliciting a form of posttranscriptional gene silencing (termed virus-induced gene silencing or VIGS), plant viruses are increasingly used as reverse-genetics tools for functional characterization of plant genes. RNA viruses have been shown to trigger silencing in a variety of host plants, including members of Solanacae and Arabidop
American Society of Plant Biologists.
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9. Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley1
We successfully implemented virus-induced gene silencing (VIGS) in barley (Hordeum vulgare) for the functional characterization of genes required for Mla13-mediated resistance toward the biotrophic barley pathogen Blumeria graminis f. sp. hordei. Initially, barley cultivars were screened for their ability to host the barley stripe mosaic virus (BSMV)-VIGS ve
American Society of Plant Biologists.
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10. CITRX thioredoxin interacts with the tomato Cf-9 resistance protein and negatively regulates defence
To identify proteins involved in tomato Cf-9 resistance protein function, a yeast two-hybrid screen was undertaken using the cytoplasmic C-terminus of Cf-9 as bait. A thioredoxin-homologous clone, interacting specifically with Cf-9, was identified and called CITRX (Cf-9-interacting thioredoxin). Virus-induced gene silencing (VIGS) of CITRX resulted in an acc
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11. Functional Analysis of Avr9/Cf-9 Rapidly Elicited Genes Identifies a Protein Kinase, ACIK1, That Is Essential for Full Cf-9–Dependent Disease Resistance in TomatoW⃞
Tomato (Lycopersicon esculentum) Cf genes confer resistance to the fungal pathogen Cladosporium fulvum through recognition of secreted avirulence (Avr) peptides. Plant defense responses, including rapid alterations in gene expression, are immediately activated upon perception of the pathogen. Previously, we identified a collection of Avr9/Cf-9 rapidly (15 to
American Society of Plant Biologists.
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12. Calcium-dependent protein kinases play an essential role in a plant defence response
Calcium-dependent protein kinases (CDPKs) comprise a large family of serine/threonine kinases in plants and protozoans. We isolated two related CDPK cDNAs (NtCDPK2 and NtCDPK3) from Nicotiana tabacum. These CDPK transcripts are elevated after race-specific defence elicitation and hypo-osmotic stress. Transiently expressed myc-epitope-tagged NtCDPK2 in Nicoti
Oxford University Press.