Viral Culture
Mostrando 1-12 de 2029 artigos, teses e dissertações.
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1. Rhino-orbito-cerebral mucormycosis caused by Rhizopus microsporus var. microsporus in a diabetic patient with COVID-19
Abstract COVID-19 disease caused by the SARS-CoV-2 coronavirus causes a wide range of clinical manifestations, ranging from mild to severe, with the main ones affecting the respiratory tract, such as pneumonia. In patients with greater severity, the high frequency of bacterial and fungal coinfection stands out, a situation related both to the patient’s pre
Anais Brasileiros de Dermatologia. Publicado em: 2022
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2. Inhibition of Brazilian ZIKV strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide
ABSTRACT Zika virus (ZIKV) infection during pregnancy is associated with a congenital syndrome. Although the virus can be detected in human placental tissue and sexual transmission has been verified, it is not clear how the virus reaches the fetus. Despite the emerging severity caused by ZIKV infection, no specific prophylactic and/or therapeutic treatment i
Braz J Infect Dis. Publicado em: 2020-12
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3. Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos
BACKGROUND The Yellow Fever (YF) vaccine is produced by the inoculation of embryonated chicken eggs with YF17DD virus on the ninth day of development. Full embryos are collected on the twelfth day of development for vaccine formulation. Skeletal muscle tissue is the main site where biosynthesis of viral particles occurs. OBJECTIVES This study aimed to an
Mem. Inst. Oswaldo Cruz. Publicado em: 09/12/2019
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4. Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus
Abstract Chrysodeixis includens has become the major Lepidopteran pest of soybean crops, especially in the Brazilian Cerrado (savanna) region. A native isolate of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) from this region, Buritis, MG, was assessed for its biological and molecular features. In addition, in vitro co-infection with Anticarsia gemm
Braz. arch. biol. technol.. Publicado em: 25/11/2019
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5. ANTICORPOS POLICLONAIS PARA O IMUNODIAGNÓSTICO DE VÍRUS BOVINO
Resumo O objetivo do estudo foi produzir anticorpos policlonais para vírus bovino e testar a reatividade em imunoensaios como imunofluorescência, imunoperoxidase e slot blot. Como imunógeno utilizou-se cepas e/ou isolados dos herpesvírus bovino tipo 1, 2 e 5 (BoHV-1, BoHV-2 e BoHV-5), vírus da diarreia viral bovina (BVDV), vírus respiratório sincicial
Ciênc. anim. bras.. Publicado em: 24/10/2019
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6. Viral etiology of acute respiratory infections in children in Southern Iran
Abstract INTRODUCTION: Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs). METHODS: Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR
Rev. Soc. Bras. Med. Trop.. Publicado em: 29/07/2019
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7. First isolation of West Nile virus in Brazil
BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molec
Mem. Inst. Oswaldo Cruz. Publicado em: 17/01/2019
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8. Low occurrence of Hepatitis A virus in water samples from an urban area of Southern Brazil
ABSTRACT Hepatitis A virus (HAV), a member of Picornaviridae family, is the main causative agent of acute viral hepatitis in the world, mainly in developing countries. HAV may be present in contaminated water and food and its presence is often associated to a lesser extent with socioeconomic factors and environmental quality. The main goals in the present st
Rev. Inst. Med. trop. S. Paulo. Publicado em: 08/11/2018
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9. Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro
Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host
Braz. J. Microbiol.. Publicado em: 2017-03
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10. Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones
Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were
Braz. J. Microbiol.. Publicado em: 2016-12
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11. Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus enc
Braz. arch. biol. technol.. Publicado em: 2015-12
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12. Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate
Braz. J. Microbiol.. Publicado em: 31/03/2015