Viral Cytopathogenic Effect
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Comparação de métodos convencionais e reação em cadeia da polimerase em tempo real na detecção de infecção pelo citomegalovirus in vitro / Comparison of conventional methods and real-time polymerase chain reaction in the detection of the cytomegalovirus infection in vitro
Introdução: Isolados clínicos do Citomegalovirus (CMV) são facilmente propagados in vitro resultando em comprometimento da monocamada celular onde o vírus foi inoculado, evidenciando assim a presença ou ausência de infecção. A cultura celular é um método clássico para detecção do CMV e foi bastante utilizada no passado. O ensaio de antigenemia,
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/09/2009
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2. Induction of cytopathogenicity in mammalian cell lines challenged with culturable enteric viruses and its enhancement by 5-iododeoxyuridine.
Cultures of 17 established cell lines were tested against 105 enteric virus types for capacity to support viral replication as indicated by cytopathogenic effect production. Enhancement of susceptibility by treatment of the cells with 5-iododeoxyuridine was evaluated in parallel with untreated cells. Cytopathogenic effect was produced in two or more cell lin
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3. Cytopathogenic effect in cardiac myocytes but not in cardiac fibroblasts is correlated with reovirus-induced acute myocarditis.
A panel of reovirus strains was used to compare myocarditic potential with induction of cytopathic effect in primary cardiac myocyte and cardiac fibroblast cultures. The results suggest that viral cytopathogenicity in cardiac myocytes, but not in cardiac fibroblasts, is a determinant of reoviral myocarditis.
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4. Classical Swine Fever Virus Leader Proteinase Npro Is Not Required for Viral Replication in Cell Culture
The sequence encoding the viral leader proteinase Npro was replaced by the murine ubiquitin gene in a full-length cDNA clone of the classical swine fever virus (CSFV) strain Alfort/187. The recombinant virus vA187-Ubi showed growth characteristics similar to those of the parent vA187-1 virus. At two occasions cells infected with vA187-Ubi exhibited a cytopat
American Society for Microbiology.
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5. Viral Susceptibility Range of the Fathead Minnow (Pimephales promelas) Poikilothermic Cell Line 1
The viral susceptibility range of a poikilothermic cell line derived from the fathead minnow (Pimephales promelas) (FHM) to infection by a number of homoiothermic viruses representing most of the presently recognized viral groups and a member of the psittacosis-lymphogranuloma-trachoma group of agents was studied. All infectious agents, except poliovirus typ
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6. Characterization of Helper Virus-Independent Cytopathogenic Classical Swine Fever Virus Generated by an In Vivo RNA Recombination System
Molecular analyses revealed that most cytopathogenic (cp) pestivirus strains evolve from noncytopathogenic (noncp) viruses by nonhomologous RNA recombination. In contrast to bovine viral diarrhea virus (BVDV), cp classical swine fever virus (CSFV) field isolates were rarely detected and always represented helper virus-dependent subgenomes. To investigate RNA
American Society for Microbiology.
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7. In Vitro Studies on the Antiviral Activity of 1,3-Bis(2-Chloroethyl)-1-Nitrosourea
Chemotherapy experiments carried out in vitro demonstrated that 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) was active against lymphocytic choriomeningitis virus and had an equivocal antiviral effect on Semliki Forest, herpes simplex, and vaccinia viruses. No antiviral effect was observed with BCNU against western equine encephalomyelitis, polio, and parainf
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8. Cytopathogenic and Noncytopathogenic RNA Replicons of Classical Swine Fever Virus
To determine the minimal requirements for autonomous RNA replication of classical swine fever virus (CSFV), genomes having in-frame deletions within the genes for structural and flanking nonstructural proteins were constructed, based on an infectious cDNA clone of CSFV Alfort/187. RNA was transcribed in vitro from the respective plasmids and transfected into
American Society for Microbiology.
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9. Cytopathogenicity of classical swine fever virus caused by defective interfering particles.
For three independent cytopathogenic isolates of classical swine fever virus, defective RNAs were found in infected cells in addition to full-length viral genomes. These RNAs represent the genomes of typical defective interfering (DI) particles because of strict dependence on a complementing helper virus and interference with the replication of the helper vi
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10. Hepatitis A Virus Inhibits Cellular Antiviral Defense Mechanisms Induced by Double-Stranded RNA†
The consequences of a hepatitis A virus (HAV) infection on cell-based antiviral responses and the interactions between virus and host cells resulting in persistent infections are poorly understood. In this report, we show that HAV does inhibit double-stranded (dsRNA)-induced beta interferon (IFN-β) gene expression by influencing the IFN-β enhanceosome, as
American Society for Microbiology.
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11. Inhibition by Rifampin of African Swine Fever Virus Replication in Tissue Culture
Vaccinia virus and African swine fever virus are deoxyribonucleic acid viruses of cytoplasmic origin. The fact that rifampin inhibits the replication of the former virus led to an investigation of its effect on African swine fever virus. The virus used was cytopathogenic to a PK-15 cell line, hemadsorbing in pig leukocyte cultures and lethal to pigs. Rifampi
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12. Use of Disposable Micro Tissue Culture Plates for Antiviral and Interferon Induction Studies
A reproducible test system requiring small amounts of test compound was developed for evaluating antiviral and interferon-inducing activity. In the antiviral experiments, KB cells were grown in disposable polystyrene microplates covered with a standard domestic plastic wrap. Viruses used in the system were types 1 and 2 herpes simplex virus, vaccinia virus,