Xprt
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. Planejamento racional de drogas contra tripanosomatídeos: gGAPDH de Trypanosoma cruzi e XPRT de Leishmania major / Rational design of anti-trypanosomatids drugs: T. cruzi gGAPDH and Leishmania major XPRT
Aiming at discover molecules with good inhibitory activity against tripanosomatides enzymatic targets, the crystallographic structures of glyceraldehydes-3-phosphate dehydrogenase in complex with 1,3 bisfosfoglyceric acid analogues (30 and 33)were solved, molecular modeling studies were undertaken and xprt (xanthine phosphorybosil transferase) gene from Leis
Publicado em: 2004
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2. Quantification of expression of linked cloned genes in a simian virus 40-transformed xeroderma pigmentosum cell line.
We wished to determine whether simian virus 40 (SV40)-transformed xeroderma pigmentosum cells, despite their defective DNA repair, were suitable for DNA-mediated gene transfer experiments with linked genes. Expression of a nonselectable gene (cat, coding for chloramphenicol acetyltransferase [CAT]) linked to a selectable gene (gpt, coding for xanthine-guanin
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3. Isolation and Characterization of the Saccharomyces cerevisiae XPT1 Gene Encoding Xanthine Phosphoribosyl Transferase
A new Saccharomyces cerevisiae gene, XPT1, was isolated as a multicopy suppressor of a hypoxanthine phosphoribosyl transferase (HPRT) defect. Disruption of XPT1 affects xanthine utilization in vivo and results in a severe reduction of xanthine phosphoribosyl transferase (XPRT) activity while HPRT is unaffected. We conclude that XPT1 encodes XPRT in yeast.
American Society for Microbiology.
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4. New Mg2+-Dependent Oxytetracycline Resistance Determinant Tet 34 in Vibrio Isolates from Marine Fish Intestinal Contents
A new oxytetracycline (OTC) resistance (Otcr) determinant, Tet 34, was cloned from chromosomal DNA of Vibrio sp. no. 6 isolated from intestinal contents of cultured yellowtail (Seriola quinqueradiata). The transformant, containing cloned Tet 34, could grow in broth containing 25 μg of drug per ml with 10 mM MgCl2. Tet 34 encoded an open reading frame (ORF)
American Society for Microbiology.