Yeast Hybrids
Mostrando 1-12 de 80 artigos, teses e dissertações.
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1. Melhoramento de leveduras para fermentação com alto teor alcoólico mediante hidridação e evolução adaptativa / Yeast improvement for high ethanol content fermentation by hybridization and adaptive evolution
O etanol contribui significativamente para que a matriz energética do país se apresente extremamente favorável quanto à participação da energia renovável. A demanda por este biocombustível é crescente e tecnologias que permitam a sua produção de forma sustentável é de suma importância, como a fermentação com alto teor de etanol, já empregada
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/06/2012
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2. Development and characterization of hybrids from native wine yeasts
For commercial purposes, the winemaking industry is constantly searching for new yeast strains. Historically, this has been achieved by collecting wild strains and selecting the best for industrial use through an enological evaluation. Furthermore, the increasing consumer demands have forced the industry to incorporate new strategies such as genetic engineer
Brazilian Journal of Microbiology. Publicado em: 2012-06
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3. Efeito dos extratos do albedo de Citrus sinensis, Lentinula edodes, Agaricus blazei e dos compostos orgânicos voláteis produzidos por Saccharomyces cerevisiae no controle da mancha preta dos citros / Effect of albedo extracts of Citrus sinensis, Lentinula edodes, Agaricus blazei and volatile organic compounds produced by Saccharomyces cerevisiae in the control of citrus black spot
The citrus black spot is a disease that restricts the export of Brazilian oranges to the countries in Europe. Except for Citrus aurantium and its hybrids, all other varieties are susceptible to the pathogen. Thus, there is great interest that this disease does not occur, since it is considered a quarantine A1 disease. Problems associated with the acquisition
Publicado em: 2010
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4. Obtaining a fluorescent and resistant hybrid yeast to the nystatin / Obtenção de levedura híbrida fluorescente e resistente a nistatina
Vats contamination by wild yeasts can harm the efficiency and the productivity of ethanol industrial production and there are not efficient methods to control this type of contamination. Easiness, speed and the low cost would be desirable characteristics for the control of yeasts that eventually contaminate the fermentation process. With this aim, TAVARES (1
Publicado em: 2007
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5. IdentificaÃÃo de leveduras dentro do complexo Saccharomyces âsensu strictoâ por PCR-fingerpriting
A relaÃÃo biolÃgica de leveduras industrialmente importantes do complexo Saccharomyces âsensu strictoâ causa equÃvoco na correta identificaÃÃo das leveduras. Por isso, este trabalho apresenta a tÃcnica de PCR - fingerprinting com diferentes marcadores que identificaram com eficiÃncia as verdadeiras espÃcies do complexo Saccharomyces âsensu strict
Publicado em: 2006
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6. Seleção de linhagens de Saccharomyces cerevisiae potencializadas pelo fator Killer, H2S- e o carater floculante. / Improvement of a saccharomyces cerevisiae strain by the characters: "Killer" skills, flocculation capacity and lack in productinof H2S-.
Flocculative and "killer" skills and lack in production of H2S are desirable characteristics of the ethanolic fermentative yeasts. Seven selected strains of Saccharomyces cerevisiae with some of these characteristics were evaluated for confirmation of these habilities and their genetic characterization was undertaken by eletrophoretic kariotyping. The strain
Publicado em: 2003
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7. A Flow Cytometric Method for Rapid Selection of Novel Industrial Yeast Hybrids
We rapidly produced and isolated novel yeast hybrids by using two-color flow cytometric cell sorting. We labeled one parent strain with a fluorescent green stain and the other parent with a fluorescent orange stain, and hybrids were selected based on their dual orange and green fluorescence. When this technique was applied to the production of hybrids by tra
American Society for Microbiology.
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8. Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.
A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapp
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9. Construction from a single parent of baker's yeast strains with high freeze tolerance and fermentative activity in both lean and sweet doughs.
From a freeze-tolerant baker's yeast (Saccharomyces cerevisiae), 2,333 spore clones were obtained. To improve the leavening ability in lean dough of the parent strain, we selected 555 of the high-maltose-fermentative spore clones by using a method in which a soft agar solution containing maltose and bromocresol purple was overlaid on yeast colonies. By measu
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10. A recombination based method to rapidly assess specificity of two-hybrid clones in yeast.
The yeast two-hybrid system is frequently used to identify protein-protein interactions. Confirming the specificity of candidate clones requires separation and isolation of yeast plasmids, propagation in bacteria and testing combinations of DNA-binding and activation domain hybrids in yeast. In order to simplify this procedure, we developed a rapid method ba
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11. Selection of Ethanol-Tolerant Yeast Hybrids in pH-Regulated Continuous Culture
Hybrids between naturally occurring wine yeast strains and laboratory strains were formed as a method of increasing genetic variability to improve the ethanol tolerance of yeast strains. The hybrids were subjected to competition experiments under continuous culture controlled by pH with increasing ethanol concentrations over a wide range to select the fastes
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12. Parasexual genetics of Torulopsis glabrata.
Prototrophic hybrids were generated in the asexual yeast Torulopsis glabrata by the fusion of spheroplasts derived from parent strains which bore complementing auxotrophic markers. The DNA content (per cell) of two hybrids was essentially that predicted by summing the corresponding parental values. UV irradiation of these two hybrids resulted in the formatio