Zinc And Calcium Binding Protein
Mostrando 1-12 de 14 artigos, teses e dissertações.
-
1. Estudos da dinâmica estrutural da proteína ligante de cálcio S100A12 humana e da lisozima T4 / Structural dynamics studies of human calcium binding protein S100A12 and T4 lysozyme
The work presented here was conceived with two main objectives. The first one, more general, involved the implementation of a new methodology for the study of conformational changes in proteins, i.e., its structural dynamics. The technique of Site-directed Spin Labeling combined with Electronic Paramagnetic Resonance (SDSL-EPR) are the pillars of this new me
Publicado em: 2011
-
2. Estudo da estabilidade estrutural de uma proteína recombinante ligante de zinco e cálcio - Calgranulina C (S100A12) porcina / Structural stability study of the zinc- and calcium- cinding recombinant protein Calgranulin C (S100A12) porcine
S100A12 porcina é um membro da família das proteínas S100, um grupo de pequenas proteínas ligantes de cálcio caracterizado pela presença de dois motivos EF-hand. Estas proteínas estão envolvidas em diversos eventos celulares, como a regulação da fosforilação protéica, atividade enzimática, tamponamento de Ca+2, processos inflamatórios e a
Publicado em: 2007
-
3. A novel method for the purification of the Xenopus transcription factor IIIA.
We have developed a quick and simple purification method that yields large quantities of the Xenopus zinc finger protein transcription factor IIIA (TFIIIA). The protein is purified in the form of the 7S storage particle (TFIIIA/5S RNA complex) found in the ovaries of immature Xenopus. Our method yields 0.5 to 1 mg of pure 7S particle per ovary. It involves a
-
4. Meeting of the minds: Metalloneurochemistry
Metalloneurochemistry is the study of metal ion function in the brain and nervous system at the molecular level. Research in this area is exemplified through discussion of several forefront areas where significant progress has been made in recent years. The structure and function of ion channels have been elucidated through high-resolution x-ray structural w
The National Academy of Sciences.
-
5. Sorption of Heavy Metals to the Filamentous Bacterium Thiothrix Strain A1
A study was undertaken to determine the ability of the filamentous bacterium Thiothrix strain A1 to sorb heavy metals from solution. Cells of Thiothrix strain A1 were harvested, washed, and suspended in solutions of metals. After an equilibration period, biomass was separated from solution and the metal content in acid-digested cells and/or filtrates was det
-
6. Clusters of charged residues in protein three-dimensional structures.
Statistically significant charge clusters (basic, acidic, or of mixed charge) in tertiary protein structures are identified by new methods from a large representative collection of protein structures. About 10% of protein structures show at least one charge cluster, mostly of mixed type involving about equally anionic and cationic residues. Positive charge c
-
7. cDNA-AFLP Reveals a Striking Overlap in Race-Specific Resistance and Wound Response Gene Expression Profiles
The tomato Cf-9 gene confers resistance to races of the fungal pathogen Cladosporium fulvum expressing the Avr9 gene. cDNA amplified fragment length polymorphism analysis was used to display transcripts whose expression is rapidly altered during the Avr9- and Cf-9–mediated defense response in tobacco cell cultures. Diphenyleneiodonium was used to abolish t
American Society of Plant Physiologists.
-
8. Energy transfer between terbium (III) and cobalt (II) in thermolysin: a new class of metal--metal distance probes.
The visible fluorescence of terbium(III) when bound to a calcium binding site of thermolysin is greatly enhanced with an excitation maximum at 280 nm but substitution of cobalt(II) for zinc at the active site decreases the intensity by 89.5%. Treatment with N-bromosuccinimide quenches enzyme tryptophan and Tb(III) fluorescence to a similar extent and suggest
-
9. Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif.
The three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa, a zinc metalloprotease, has been solved to a resolution of 1.64 A by multiple isomorphous replacement and non-crystallographic symmetry averaging between different crystal forms. The molecule is elongated with overall dimensions of 90 x 35 x 25 A; it has two distinct structur
-
10. Condensation by DNA looping facilitates transfer of large DNA molecules into mammalian cells
Experimental studies of complete mammalian genes and other genetic domains are impeded by the difficulty of introducing large DNA molecules into cells in culture. Previously we have shown that GST–Z2, a protein that contains three zinc fingers and a proline-rich multimerization domain from the polydactyl zinc finger protein RIP60 fused to glutathione S
Oxford University Press.
-
11. Chemical Constituents and Hydrogenase Binding in Cell Envelopes of Vibrio succinogenes
The particulate hydrogenase of Vibrio succinogenes is solubilized during treatment of cell envelopes at pH 11.0. Alkali-solubilized enzyme requires sulfhydryl compounds for activity. At neutral pH, soluble enzyme is reincorporated into alkalitreated cell envelopes and no longer requires an additional activator. In the present study, cell envelopes prepared b
-
12. Induction of endonuclease-mediated apoptosis in tumor cells by C-nitroso-substituted ligands of poly(ADP-ribose) polymerase.
6-Nitroso-1,2-benzopyrone and 3-nitrosobenzamide, two C-nitroso compounds that inactivate the eukaryotic nuclear protein poly(ADP-ribose) polymerase [NAD+:poly(adenosine diphosphate D-ribose) ADP-D-ribosyltransferase, ADPRT, EC 2.4.2.30] at one zinc-finger site, completely suppressed the proliferation of leukemic and other malignant human cells and subsequen